Cow Spring has an oval shaped pool 66 ft (20.1 m) long and 18 ft (5.5 m) wide. The circular vent is in the center of the spring pool and is approximately 25 ft (7.6 m) deep. The pool bottom is limestone with aquatic vegetation and a small amount of algae. There is no boil present on the blue-green water surface. The spring is currently flooded by the Suwannee River, however a discernable dry spring run bed leads 0.1 miles (0.2 km) southwest to the Suwannee River. The run has abundant vegetation including shrubs and small trees growing in its dry bed. The northwest, northeast and southeast banks of the pool have exposed limestone extending up to 6 ft (1.8 m) above the water surface. The southwest side of the pool slopes gently up into the surrounding woodlands. The spring is owned by the National Speleological Society and has an extensive, mapped cave system.
From the intersection of SR 51 and 180th Street in Luraville, drive south on SR 51 approximately 1.6 miles (2.6 km) to the bridge over the Suwannee River. Launch boat at the bridge boat landing and boat approximately 4.4 miles (7.1 km) downriver. Cow Spring is located inland from Running Springs approximately 0.1 miles (0.2 km) northeast.