Little Otter Spring, also known as GIL94972, has a circular spring pool with an 87 ft (26.5 m) diameter. The vent is located in the center of the pool, and depth over the vent is 24.0 ft (7.3 m). The bottom is soft sand and mud with a thick layer of algae and tree debris around the pool edge. Spring water is bluish and slightly turbid. The spring run is 6 ft (1.8 m) wide, 3 ft (0.9 m) deep and flows west approximately 150 ft (45.7 m) into the east side of the Suwannee River. The run bottom has some exposed limestone; however, it is mostly soft sand and detritus with a thin layer of algae. Higher ground gently slopes to 3 ft (0.9 m) above water level around the spring pool. Little Otter Spring is surrounded by privately-owned, forested floodplain of the Suwannee River. Discharge on September 4, 1997 was 15.3 ft3/s.
At the junction of US 26 and CR 232 in Wilcox, drive north on CR 232 for approximately 1.7 miles (2.7 km) to the intersection with CR 334. Turn west (left) onto CR 334 and drive approximately 2.3 miles (3.7 km) to the boat landing. The spring is located along the east side of the Suwannee River approximately 0.3 miles (0.5 km) downstream from the Otter Spring Run mouth.