The Rock Bluff Springs pool measures 250 ft north to south and 171 ft east to west. Numerous vents feed the large shallow spring pool, and the most prominent vent discharges from a deep vertical limestone fissure on the north end of the pool. The depth over the fissure measures 27.8 ft (8.5 m). The water is clear with a greenish tint, and the bottom is exposed limestone and sand. Water quality was sampled from a smaller, circular vent opening in limestone 20 ft south of the main vent. The circular opening measures approximately 1.5 ft in diameter. Discharge over the sampled vent creates a prominent boil on the pool surface. A rock retaining wall is constructed along the north shore of the spring pool. There are numerous cypress trees both in the spring pool and along the perimeter of the spring.
The spring run flows southwest approximately 750 ft before entering the Suwannee River from the east about 0.25 miles upstream from Rock Bluff. The surrounding land is forested lowland floodplain. There is a cleared sandy area on the north shore above the rock retaining wall. An underwater cave system has been mapped at this spring.
Rock Bluff Springs are located 11 miles south of Branford. The springs can be accessed by boat. From the bridge over the Suwannee River in Branford head west on US 27 approximately 1.3 miles to the intersection with CR 349. Turn south (left) on CR 349 and travel approximately 10.5 miles to the intersection with CR 340. Turn east (left) onto CR 340 and travel approximately 3.3 miles to Rock Bluff boat landing on the east side of the river. The 750 ft spring run flows into the east side of the Suwannee River approximately 0.25 miles upstream from Rock Bluff Landing.