Rossetter Spring emerges directly from a small cave at the base of 25 ft high limestone and sand banks. The small, circular pool is 15 ft in diameter. The depth of the spring measured near the cave opening is 4.5 ft. The water was slightly turbid, greenish colored, and had abundant filamentous algae in August 2002. The shallow run is L-shaped and it flows 60 ft south then turns sharply west, flowing another 60 ft until reaching the river. It has a sand and rock bottom with some detritus deposition. There is a 3 ft high, man-made, limestone wall at the mouth of the spring run intended to capture water for swimming.
State-owned land around the spring supports a well managed pine forest.
Rossetter Spring is on the east side of the Withlacoochee River approximately 11 miles (18 km) northeast of Madison.
From the intersection of SR 53 and SR 6 in Madison, travel east on SR 6 approximately 11.6 miles (18.6 km) to the intersection with CR 143, which is approximately 1.5 miles (2.4 km) past the bridge over the Withlacoochee River. Turn north (left) onto CR 143 and travel approximately 4.1 miles (6.6 km) to the intersection with Florida Campsite Road. Turn west (left) and travel approximately 3 miles to the boat landing. The spring can be accessed by boating approximately 4.2 miles (6.8 km) upstream from the boat ramp off CR 143 and Florida Campsite Road.