This spring, formerly named COL61981 (Hornsby and Ceryak, 1998), is a large circular depression with steep sides. Spring pool diameter measures 192 ft (58.5 m) north to south and 215 ft (65.5 m) northeast to southwest. Spring depth is 83 ft (25.3 m). The water color is typically clear and tinged greenish blue though it was tannic in October 2001. No boil was observed during the October 2001 visit. The spring run is approximately 90 ft (27.4 m) long and flows southeasterly into the Santa Fe River. Some algae are present on limestone substrate in the spring run. No other aquatic vegetation could be seen through the dark water. Very little emergent vegetation is present. Cypress trees are common along the water line. The spring pool is surrounded by 15-20 ft (4.6- 6.1 m) high steep, sandy banks. The uplands around the pool are generally forested with live oaks and pines.
Santa Fe Spring is located approximately 8 miles (12.9 km) northeast of High Springs on the west bank of the Santa Fe River. From the intersection of US 441/41 and CR 236 in High Springs head north on US 441/41 approximately 6.2 miles (10 km) to the O'Leno State Park sign on the east (right) side of US 441/41. Turn east (right) onto an access road, which parallels US 441/41 and travel 0.3 miles (0.5 km) to a dirt road on the east (right) side of the road, just past the O'Leno State Park entrance. Turn east (right) onto the dirt road and travel approximately 3.3 miles (5.3 km) to a boat landing just upstream from the I-75 bridge. The road makes a series of 90 degree turns to the north and east before finally bearing southeast to the Santa Fe River. The spring is 2 miles (3.2 km) upstream from the I-75 bridge over the river. At this point, a narrow spring run comes in from the north. The spring is approximately 90 ft (27.4 m) up the spring run at the head.