Treehouse Spring is in a circular cove on the southeast side of the Santa Fe River. The spring discharges westward into the adjacent river. Spring pool diameter measures 125 ft (38.1 m) north to south and 175 ft (53.3 m) east to west. Pool depth over the vent is 31 ft (9.4 m). Water color was tannic, and there was no spring boil during October 2001. Water hyacinth was the only non-native plant species observed in the spring pool. No other vegetation could be seen through the dark water. Land adjacent to this spring is a forested lowland flood plain. The nearest high ground is approximately 150 ft (46 m) to the east, and it rises 10-12 ft (3-3.7 m) higher than the flood plain and is forested with mixed hardwoods and pines. Treehouse Spring is also published as ALA112971 (Hornsby and Ceryak, 1998).
From downtown High Springs drive about two miles north on U.S. 41/441 to bridge over the Santa Fe. The boat ramp to the river is on the NW side of the bridge. Put in and go upriver about 2/3 mile. Look for a circular cove on the right, created by the spring.