Twin Spring occupies an oval-shaped pool that measures 130 ft (39.6 m) east to west and 36 ft (11 m) north to south. The vent is a fissure in the limestone bottom near the center of the pool. The pool bottom is sand with some limestone and abundant Hydrilla. The water is clear and bluish. The 200 ft (61 m) long spring run flows north to the Santa Fe River. The run averages 10 ft (3.1 m) wide, 4 ft (1.2 m) deep and is completely covered with aquatic vegetation. The spring is located within Ginnie Springs Outdoors, a private campground and outdoor recreation concession and the pool is a take-out point for people tubing in the spring run. A wooden platform is located on the north side of the pool. To the west is a picnic area with tables and barbecue pits. A mixed hardwood forest surrounds the spring area. Discharge on November 4, 1997 measured 19.55 ft3/s.
From High Springs, drive south on U.S. 27/41 about 1.0 mile (1.6 km). Turn west (right) onto State Road 340 (Poe Springs Road) and drive about 6.6 miles (10.6 km) and then turn north (right) onto a graded road at the sign for Ginnie Springs. Follow the road another mile (1.6 km) to the entrance of Ginnie Springs. Twin Spring is the second most downstream spring in the Ginnie Springs complex.